Journal: bioRxiv

Article Title: Plastin-3 membrane recruitment drives cell-in-cell invasion during entosis

doi: 10.64898/2026.03.17.709257

Figure Lengend Snippet: (A) Representative immunoblot images of ROCK1, RhoA, pMCL2 and tubulin (loading control) in MCF7 treated with 1uM narciclasine at different time-points. (B) Densitometric quantification of ROCK1, RhoA and pMCL2 protein levels from immunoblot images. (C) Immunofluorescence staining for pMCL2 (green) and actin (red) in MCF7 cells treated with 1 µM narciclasine for 6 hours. Nuclei were counterstained with DAPI (blue). (D) Summary of quantification of entotic events in MCF7 cells treated with vehicle, 1 µM narciclasine, or 50 µM blebbistatin (1 hour pretreatment) in combination with 1 µM narciclasine for 24 hours. (E) Representative 3D confocal images of entotic structures following 6 hours of treatment with vehicle or 1 µM narciclasine. White arrows indicate entotic events. Selected regions (boxed) are magnified, with corresponding orthogonal z-stack views shown adjacent to each image. (F) Summary of quantification of entotic events from the conditions shown in panel E. For all confocal microscopy panels, orthogonal z-stack views and magnified insets are provided. Experiments were performed in biological triplicate (n = 3). Data are presented as mean ± SEM. Data in panel B are normalized to untreated controls within each experiment (set to 100%). Statistical analysis for panels B and D was performed using one-way ANOVA followed by Bonferroni’s post hoc test (**p < 0.01, ***p < 0.001, *p < 0.0001), whereas panel F was analyzed using a paired two-tailed Student’s t-test (*p < 0.05).

Article Snippet: Narciclasine, Y27632, zVAD-fmk, and Blebbistatin were obtained from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Western Blot, Control, Immunofluorescence, Staining, Confocal Microscopy, Two Tailed Test